The quantity of spike protein created via the mRNA transfection process in humans continues to grow. This case shows that at 3.5 years, the lack of an off switch allows continual gene expression. This means that individuals may express that gene and produce those proteins indefinitely.
This case documents the longest reported in vivo persistence of vaccine-derived mRNA, plasmid DNA fragments, and spike protein following mRNA vaccination, with reproducible detection across multiple independent laboratories, distinct biological compartments, and complementary molecular detection systems extending beyond 3.5 years after the final dose. Spike protein, spike mRNA sequences, and plasmid backbone elements were identified in both immune cells and somatic tissue, with continued absence of SARS-CoV-2 nucleocapsid protein or antibodies, effectively excluding prior infection as the source. [via Persistence of Vaccine mRNA, Plasmid DNA, Spike Protein, and Genomic Dysregulation Over 3.5 Years Post-COVID-19 mRNA Vaccination | Medical Research Archives, archived 17 July 2026]